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Human IL-17

北京索萊寶提供高品質(zhì)的Human IL-17 ELISA KIT，也可以幫客戶開發(fā)定制ELISA KIT。我們注重技術(shù)的掌握和優(yōu)化、自有產(chǎn)品的研發(fā)和生產(chǎn)，同時(shí)強(qiáng)調(diào)良好的售后服務(wù)和技術(shù)支持，我們?yōu)閺V大科研學(xué)者而服務(wù)，一直在路上。

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PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IL-17 is added to detect the captured IL-17 protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.